Coding

Part:BBa_K759005:Design

Designed by: Ami Kawahata   Group: iGEM12_HokkaidoU_Japan   (2012-09-19)


phaB (acetoacetyl-CoA reductase orgin from Ralstonia eutropha)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 472
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 540


Design Notes

We cloned the gene by constructing primers.


Source

This gene was cloned from pGEM phbCAB. The pGEM phbCAB carries the synthetic operon genes (phaA,phaB,phaC) of Ralstonia eutropha.

References